Fibrogenic and fibrolytic potential of differently activated human macrophages

Macrophages are involved in the regulation of fibrogenesis and turnover extracellular matrix. One way to perform this function is through production profibrotic fibrolytic factors including fibronectin, laminin, collagen, matrix proteases. The most them has been well studied experimental models; however, much remains unclear regarding human macrophages. Therefore, aim study was content proteases (MMP-2 MMP-9, cathepsin L), their inhibitors (TIMP-1), collagen (type I) supernatants differently activated We compared macrophages differentiated by M-CSF or GM-CSF further polarized M1 with lipopolysaccharide, M2a IL-4, M2c dexamethasone. obtained from peripheral blood monocytes. MMPs, TIMP, cathepsin, determined using appropriate ELISA kits. results demonstrate that differentiation more important for above polarizing stimuli (lipopolysaccharide, dexamethasone). Moreover, showed predominantly antifibrotic activity because pronounced MMPs production, while GM-CSF-induced cultures, on contrary, were characterized properties due high level TIMP-1 type I collagen. M1, M2a, M2c, induced M-CSF, differed only MMP-2 produced metalloproteinase than other subtypes. In case GM-CSF-differentiated cells, a higher and, lesser extent, characteristic whereas have minimal concentration among macrophage Concerning L relatively constant did not depend generation conditions (differentiation signals). Thus, data help identify subtypes anti- potential may be useful development cell therapy diseases associated dysregulation.